Features
- Sensitivity to handle low-input samples—Works with as little as 200 pg of input RNA rRNA-depleted material (derived from 2–100 ng of total RNA)
- Efficient results from compromised samples—Ideal solution for FFPE samples
- RNA-seq data quality—Consistent RNA-seq data across all input ranges (200 pg–10 ng)
- Mappability > 80%
- Reproducibility >0.98
- ERCC correlation > 0.99
- Number of genes > 15,000
- Complete kit includes RT and PCR enzymes
- Use the table below to choose the right kit:
| cDNA Synthesis Kit |
RNA-Seq Library Preparation |
SMARTer Universal Low Input RNA Kit for Sequencing (Cat. Nos. 634938, 634940)
|
Ion Torrent or Illumina kits (not included) |
| SMARTer Universal Low Input RNA Library Prep Kit (Cat. Nos. 634945, 634946) |
Low Input Library Prep Kit included (for Illumina sequencing only) |
Interested in more data and FAQs about this product? Visit the NGS Resource Portal
Applications
- Generate cDNA for RNA-seq from very low amounts of total RNA (2–10 ng) from FFPE or LCM samples
- Generate cDNA for NGS from very low amounts of total RNA (2–10 ng) that are:
- Compromised (obtained from FFPE or LCM samples)
- Have low RIN values (<8)
- Are from sources that do not have polyadenylated mRNA
Additional Information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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