Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Gel stability tested for a period of 14 days at 37°C
• Biological activity determined for each lot using neurite outgrowth assay.

Source

EHS mouse tumor

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.