从细胞、组织、血液中提出高纯度的RNA，得率大概10-50mg组织可以提取50ugRNA。RNA提取试剂盒，优点有：时间短，15分钟完成；试剂盒中包含了苯酚等试剂，无需额外提供；不需要氯仿和苯酚分层提取过程；RNA质量高，得率高。
GENEzol™ TriRNA Pure Kit (GZX050/GZX100/GZX200)
The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
Advantages (Cat. # GZX050, GZX100, GZX200)
Purify total RNA in 15 minutes!
A cost effective phenol, guanidine isothiocyanate solution plus spin column system
No chlorophorm phase separation
No isopropanol RNA precipitation
No phenol carryover
Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
High quality RNA: A260/A280 >1.8, A260/A230 >1.8
Binding Capacity: 50 μg RNA from ≥ 25 μl DNase/RNase-free Water
Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
Storage: GENEzol™ Reagent should be stored at 2oC to 25oC, other components should be stored at room temperature (15-25oC)
Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

Components
GENEzol™ Reagent
Pre-Wash Buffer
Wash Buffer
RNase-free Water
RB Columns
2 ml Collection Tubes
Quality Control
The GENEzol™ TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.
GENEzol™ TriRNA Pure Kit Interactive Protocol

GENEzol™ TriRNA Pure Kit Functional Test Data

Figure 1. RNA was purified using the GENEzol™ TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 μl from a 50 μl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

Table 1. Total RNA purified using the GENEzol™ TriRNA Pure Kit and competitor Z.

Table 2. RNA was purified using the GENEzol™ TriRNA Pure Kit. RNA from 200 μl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 μl from each 35 μl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.