Biolog 1005 YT鉴定板 用于酵母菌的鉴定
美国BIOLOG公司*研发的产品 ECO板，特别为微生物群落分析和生态研究设计，Eco板上包含国际土壤微生物学家精选的用于微生物群落分析最常用的31种碳源，是国际上微生物生态功能多样性研究的最典型和*方法之一。
每块96孔ECO板上有3组平行，每组31种碳源，3个阴性对照，加入同一样品后，可以获得3组平行数据。微生物群落对这31种碳源的特征性利用称作该微生物群落的代谢指纹图谱。从单个的微平板上的指纹图谱就可以获得大量的代谢信息。可用于纯种及混合菌群(如土壤样品)微生物功能多样性研究。
31种碳源可分为六大类，具体如下：
单糖\糖苷\聚合糖类
B2， D-木糖
H1, a-D-乳糖
A2, ?-甲基D-葡萄糖苷
G2,葡萄糖-1-磷酸盐
E1, a-环状糊精
F1, 肝糖
G1, D-纤维二糖
氨基酸类，
A4，L-精氨酸，
B4，L-天冬酰胺酸
C4 L-苯基丙氨酸
D4, L-丝氨酸
E4，L-苏氨酸
F4，甘氨酰-L-谷氨酸
酯类
B1, 丙酮酸甲酯
C1, 吐温40
D1, 吐温80
A3, D-半乳糖酸γ内酯
醇类
C2, I-赤藻糖醇
D2, D-甘露醇
H2, D,L-a-甘油
胺类
G4, 苯乙基胺
H4, 腐胺
E2, N-乙酰基-D-葡萄胺
酸类
B3,D-半乳糖醛酸
F2 D-氨基葡萄糖酸
C3,2-羟苯甲酸
D3,4-羟基苯甲酸
E3,r-羟基丁酸
F3,衣康酸
G3,a-丁酮酸
H3,D-苹果酸
样品简单处理后(过滤或离心)，取上清液加到ECO板上培养多至5天时间，每隔一定时间用酶标仪在590nm和750nm下测定OD值，OD值的大小反应样品微生物利用某一碳源底物的能力，将OD值导出后进行分析，如AWCD值(平均吸光度)、丰富度、单孔的动力学分析、不同类碳源的比较等，并可用聚类软件对不同样品进行聚类分析。
值得一提的是ECO板不仅测定可培养微生物，还可测定部分不可培养微生物，只要微生物能利用碳源进行代谢(并不增殖)，ECO板上显色体系均可检测出来。
最常见的应用是利用ECO板分析环境因素、根系、叶面、肥料、农药对土壤微生物菌群的生态功能多样性的影响，比较不同样品间或同一样品不同时间的功能多样性差异，比较环境修复前后微生物群落的功能多样性差异，等等。
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BIOLOG 鉴定板与培养基
Microplate Panels and Media
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GEN II & GEN III MicroPlate Consumables
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· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
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Catalog Number
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Description
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1030
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GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria)
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1011
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GN2 MicroPlate (GEN II for Gram-negative bacteria)
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1014
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GP2 MicroPlate (GEN II for Gram-positive bacteria)
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1007
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AN MicroPlate (GEN II for Anaerobic bacteria)
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1005
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YT MicroPlate (GEN II for Yeasts)
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1006
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FF MicroPlate (GEN II for Filamentous Fungi)
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Special Purpose MicroPlates
· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
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Catalog Number
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Description
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1013
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MT2 MicroPlate (for metabolic studies)
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1511
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SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes)
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1514
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SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes)
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1506
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ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis)
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Biolog Prepared Plated Media
· Sold in increments of 15 plates/pack
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Catalog Number
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Description
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71102
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BUG (Biolog Universal Growth) Agar w/ 5% sheep blood
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71103
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BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1
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71005
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BUY (Biolog Universal Yeast) Agar1
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Other Prepared Plated Media
· Sold in increments of 10 plates/pack
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Catalog Number
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Description
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Bio-M1040
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Tryptic Soy Agar (TSA)
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Bio-M1006
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Tryptic Soy Agar (TSA) w/ 5% sheep blood
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Bio-M1012
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Chocolate Agar, enriched
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71007
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BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood
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Bio-M1065
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R2A Agar
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71106
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2% Malt Extract Agar
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Biolog Dehydrated Media
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Catalog Number
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Description
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70101
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BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates)
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70007
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BUA (Biolog Universal Anaerobe) Agar
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70005
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BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates)
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GEN III Starter Kits
· Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications
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Catalog Number
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Description
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39993A-O
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20 GEN III MicroPlates
·1 box of pipette tips (Ovation)
·1 CASE OF reservoirs
·1 package OF Inoculatorz (100 units individually packaged)
·20 tubes OF IF-A
·20 tubes OF IF-B
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39993A-M
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20 GEN III MicroPlates
· 1 box of pipette tips (Matrix)
· 1 CASE OF reservoirs
· 1 package OF Inoculatorz (100 units individually packaged)
· 20 tubes OF IF-A
· 20 tubes OF IF-B
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39993B
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30 BUG+blood plated media for GEN III
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