美国BIOLOG公司*研发的产品 ECO板,特别为微生物群落分析和生态研究设计,Eco板上包含国际土壤微生物学家精选的用于微生物群落分析最常用的31种碳源,是国际上微生物生态功能多样性研究的最典型和*方法之一。
每块96孔ECO板上有3组平行,每组31种碳源,3个阴性对照,加入同一样品后,可以获得3组平行数据。微生物群落对这31种碳源的特征性利用称作该微生物群落的代谢指纹图谱。从单个的微平板上的指纹图谱就可以获得大量的代谢信息。可用于纯种及混合菌群(如土壤样品)微生物功能多样性研究。
31种碳源可分为六大类,具体如下:
单糖\糖苷\聚合糖类
B2, D-木糖
H1, a-D-乳糖
A2, ?-甲基D-葡萄糖苷
G2,葡萄糖-1-磷酸盐
E1, a-环状糊精
F1, 肝糖
G1, D-纤维二糖
氨基酸类,
A4,L-精氨酸,
B4,L-天冬酰胺酸
C4 L-苯基丙氨酸
D4, L-丝氨酸
E4,L-苏氨酸
F4,甘氨酰-L-谷氨酸
酯类
B1, 丙酮酸甲酯
C1, 吐温40
D1, 吐温80
A3, D-半乳糖酸γ内酯
醇类
C2, I-赤藻糖醇
D2, D-甘露醇
H2, D,L-a-甘油
胺类
G4, 苯乙基胺
H4, 腐胺
E2, N-乙酰基-D-葡萄胺
酸类
B3,D-半乳糖醛酸
F2 D-氨基葡萄糖酸
C3, 2-羟苯甲酸
D3, 4-羟基苯甲酸
E3,r-羟基丁酸
F3, 衣康酸
G3,a-丁酮酸
H3, D-苹果酸
样品简单处理后(过滤或离心),取上清液加到ECO板上培养多至5天时间,每隔一定时间用酶标仪在590nm和750nm下测定OD值,OD值的大小反应样品微生物利用某一碳源底物的能力,将OD值导出后进行分析,如AWCD值(平均吸光度)、丰富度、单孔的动力学分析、不同类碳源的比较等,并可用聚类软件对不同样品进行聚类分析。
值得一提的是ECO板不仅测定可培养微生物,还可测定部分不可培养微生物,只要微生物能利用碳源进行代谢(并不增殖),ECO板上显色体系均可检测出来。
最常见的应用是利用ECO板分析环境因素、根系、叶面、肥料、农药对土壤微生物菌群的生态功能多样性的影响,比较不同样品间或同一样品不同时间的功能多样性差异,比较环境修复前后微生物群落的功能多样性差异,等等。
BIOLOG 鉴定板与培养基
Microplate Panels and Media
GEN II & GEN III MicroPlate Consumables |
|
· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
Catalog Number | Description |
1030 | GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria) |
1011 | GN2 MicroPlate (GEN II for Gram-negative bacteria) |
1014 | GP2 MicroPlate (GEN II for Gram-positive bacteria) |
1007 | AN MicroPlate (GEN II for Anaerobic bacteria) |
1005 | YT MicroPlate (GEN II for Yeasts) |
1006 | FF MicroPlate (GEN II for Filamentous Fungi) |
Special Purpose MicroPlates
· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
Catalog Number | Description |
1013 | MT2 MicroPlate (for metabolic studies) |
1511 | SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes) |
1514 | SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes) |
1506 | ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis) |
Biolog Prepared Plated Media
· Sold in increments of 15 plates/pack
Catalog Number | Description |
71102 | BUG (Biolog Universal Growth) Agar w/ 5% sheep blood |
71103 | BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1 |
71005 | BUY (Biolog Universal Yeast) Agar1 |
Other Prepared Plated Media
· Sold in increments of 10 plates/pack
Catalog Number | Description |
Bio-M1040 | Tryptic Soy Agar (TSA) |
Bio-M1006 | Tryptic Soy Agar (TSA) w/ 5% sheep blood |
Bio-M1012 | Chocolate Agar, enriched |
71007 | BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood |
Bio-M1065 | R2A Agar |
71106 | 2% Malt Extract Agar |
Biolog Dehydrated Media
Catalog Number | Description |
70101 | BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates) |
70007 | BUA (Biolog Universal Anaerobe) Agar |
70005 | BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates) |
GEN III Starter Kits
· Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications
Catalog Number | Description |
39993A-O | 20 GEN III MicroPlates |
· 1 box of pipette tips (Ovation) | |
· 1 CASE OF reservoirs | |
· 1 package OF Inoculatorz (100 units individually packaged) | |
· 20 tubes OF IF-A | |
· 20 tubes OF IF-B | |
39993A-M | 20 GEN III MicroPlates |
· 1 box of pipette tips (Matrix) | |
· 1 CASE OF reservoirs | |
· 1 package OF Inoculatorz (100 units individually packaged) | |
· 20 tubes OF IF-A | |
· 20 tubes OF IF-B | |
39993B | 30 BUG+blood plated media for GEN III |